Journal: Cell reports

Article Title: Systematic Single Cell Pathway Analysis (SCPA) to characterize early T cell activation

doi: 10.1016/j.celrep.2022.111697

Figure Lengend Snippet: Key resources table

Article Snippet: Human bulk CD4 + or CD8 + T cells were isolated from PBMCs of one donor (male aged 39 years old) by centrifugation using Lymphoprep separation medium (Corning, Vienna, VA) and subsequent use of either the MACS Human CD8 + T cell Isolation Kit (Miltenyi Biotech), or the Negative Selection EasySep CD4 + T cell kit (Stemcell Technologies) according to the manufacturers’ instructions.

Techniques: Generated, Virus, Recombinant, Lysis, Enzyme-linked Immunosorbent Assay, Cell Isolation, Staining, CRISPR, Control, Software

Journal: Cell reports

Article Title: Systematic Single Cell Pathway Analysis (SCPA) to characterize early T cell activation

doi: 10.1016/j.celrep.2022.111697

Figure Lengend Snippet: Key resources table

Article Snippet: Human bulk CD4 + or CD8 + T cells were isolated from PBMCs of one donor (male aged 39 years old) by centrifugation using Lymphoprep separation medium (Corning, Vienna, VA) and subsequent use of either the MACS Human CD8 + T cell Isolation Kit (Miltenyi Biotech), or the Negative Selection EasySep CD4 + T cell kit (Stemcell Technologies) according to the manufacturers’ instructions.

Techniques: Generated, Virus, Recombinant, Lysis, Enzyme-linked Immunosorbent Assay, Cell Isolation, Staining, CRISPR, Control, Software

Journal: eLife

Article Title: A dynamic interaction between CD19 and the tetraspanin CD81 controls B cell co-receptor trafficking

doi: 10.7554/eLife.52337

Figure Lengend Snippet:

Article Snippet: The diluted collar blood was then layered on top of 10 mL Lymphoprep density gradient medium (Stemcell Technologies).

Techniques: Western Blot, Flow Cytometry, Recombinant, Functional Assay, Activation Assay, DNA Extraction, Clone Assay, CRISPR, Sequencing, Knock-Out, Expressing, Plasmid Preparation, Software